Coding
Part:BBa_K5349002:Design
Designed by: Tomoharu Sakata Group: iGEM24_BotchanLab-Tokyo (2024-09-26)
scpB, biotin-independent methylmalonyl-CoA carboxylase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 172
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 631
Design Notes
The DNA was synthesized using the Integrated DNA Technologies (IDT) DNA synthesis service. Additionally, certain codons were modified to reduce synthesis difficulty while maintaining the original sequence's functionality.
Source
The sequence data was obtained from the Escherichia coli K12 MG1655 genome available in the National Center for Biotechnology Information (NCBI) database.